KMID : 0545120150250071047
|
|
Journal of Microbiology and Biotechnology 2015 Volume.25 No. 7 p.1047 ~ p.1055
|
|
A Novel Synthetic Compound, YH-1118, Inhibited LPS-Induced Inflammatory Response by Suppressing I¥êB Kinase/NF-¥êB Pathway in Raw 264.7 Cells
|
|
Yun Chang-Hyun
Jang Eun-Jung Kwon Soon-Cheon Lee Mee-Young Lee Sang-Ku Oh Sei-Ryang Lee Hyeong-Kyu Ahn Kyung-Seop Lee Ho-Jae
|
|
Abstract
|
|
|
For the search of a potent first-in-class compound to inactivate macrophages responsible for inflammatory responses, in the present study, we investigated the anti-inflammatory effects of YH-1118, a novel synthetic compound, in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line, Raw 264.7. YH-1118 inhibited LPS-induced nitric oxide (NO) production and inducible NO synthase (iNOS) expression at both the protein and mRNA levels. The suppression of LPS-induced iNOS expression by YH-1118 was mediated via nuclear factor kappa B (NF-¥êB), but not activator protein-1 (AP-1) transcription factor. This was supported by the finding that YH-1118 attenuated the phosphorylation of inhibitor of ¥êB¥á (I¥êB¥á) and nuclear translocation and DNA binding activity of NF-¥êB. Through the mechanisms that YH- 1118 inhibited the activation of I¥êB kinases (IKKs), upstream activators of NF-¥êB, or p38 MAPK, YH-1118 significantly suppressed LPS-induced production of pro-inflammatory cytokines, tumor necrosis factor-¥á, interleukin-1¥â (IL-1¥â), and IL-6 (p < 0.05). In conclusion, our results suggest that YH-1118 inhibits LPS-induced inflammatory responses by blocking IKK and NF-¥êB activation in macrophages, and may be a therapeutic candidate for the treatment of various inflammatory diseases.
|
|
KEYWORD
|
|
YH-1118, iNOS, NF-¥êB, macrophages, inflammation
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|